Nitro Blue Tetrazolium (NBT)
Name: Nitro Blue Tetrazolium (NBT)
CAS Registry Number: 298-83-9
CA Index Name: 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy [1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl]-, chloride (1:2)
Other Names: 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy [1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phe-nyl]-, dichloride; 2H-Tetrazolium, 3,3'-(3,3'-dimethoxy-4,4'-biphenylylene)bis[2-(p-nitrophenyl)-5-phenyl]-, dichloride; Tetrazolium, 3,3'-(3,3'-dimethoxy-4,4'-biphe-nylylene)bis[2-(p-nitrophenyl)-5-phenyl-2H-chloride]; 2, 2'-Bis(p-nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-biphenylylene)ditetrazolium chloride; 2,2'-Bis(p-ni-trophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride; 2,2'-Di-p-nitrophenyl-5,5'-diphenyl-3,3'-bis(3,3'-dimethoxy-4,4' -biphenylene) ditetrazolium chloride; 2,2'-Dinitrophenyl-5,5'-diphenyl-3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride; 3,3'-(3,3'-Dimethoxy-4,4'-biphenylylene)bis[2-(p-nitro-phenyl)-5-phenyl-2H-tetrazolium chloride]; 3,3'-(3,3'-Dimethoxy-4,4'-diphenylene)bis[2-(p-nitrophenyl)-5-phenyltetrazolium chloride]; NBT; NBT (dye); NSC 27622; NTB; Nitro BT; Nitro Blue Tetrazolium; Nitro Blue Tetrazolium chloride; Nitro Tetrazolium BT; Nitro-tetrazolium Chloride Blue; Nitrotetrazolium blue; Tetra-zolium Nitro BT; Tetrazolium nitro blue; p-NBT; p-Nitro blue tetrazolium; p-Nitro blue tetrazolium chloride; p-Nitrotetrazolium blue
Merck Index Number: Not listed
Chemical / Dye Class: Tetrazolium salt
Molecular Formula: C40H30Cl2N10O6
Molecular Weight: 817.64
Physical Form: Yellow powder
Solubility: Soluble in water, ethanol, methanol, dimethyl sulfoxide
Melting Point: 189 °C
Absorption (λmax): 256 nm
Synthesis: Synthetic methods
Staining Applications: Antibody; antigen; cells; dihydrolipoamide dehydrogenase (DLDH) diaphorase; enzymes; gene expression
Biological Applications: Diagnosis of Alzheimer’s disease, bacterial vaginosis, behavioral disturbances in children, Hirschsprung disease; phosphatase assay; phytase assay; detecting bacteria, microorganisms, phosphoinositides, yeast; generating and detecting reactive oxygen species; treating cancer, neuropathic pain
Industrial Applications: Microchip
Safety / Toxicity: Carcinogenicity; chronic toxicity; cytotoxicity; hepatotoxicity; mitochondrial damage; mutagenicity; nephrotoxicity; neurotoxicity; ototoxicity; phototoxicity; pulmonary toxicity
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